col ii protein expression (Micromass UK Limited)
Structured Review

Col Ii Protein Expression, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/col ii protein expression/product/Micromass UK Limited
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis"
Article Title: A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis
Journal: Bone Marrow Research
doi: 10.1155/2016/3152065
Figure Legend Snippet: List of antibodies used for IHC of micromass cartilages.
Techniques Used: Plasmid Preparation
Figure Legend Snippet: IHC of COL II (a) and COL I (b) in micromass cartilages. COL II expressed at weeks 2–4 (a), and COL I expressed at weeks 1–4 (week 3 not shown, b), in micromass cartilages. Articular cartilage of rat knee and human tracheal cartilage served as positive control for COL II; higher magnification of week 4 micromass cartilage shows that it is present in ECM (a). COL I is absent in native articular cartilage in positive control and is present in bone only, whereas COL I is expressed at low level in the bioengineered micromass cartilage (b). Micromass cartilage and rat tibia showed no staining when primary antibody was missing and only mouse IgG was present, which served as negative control (c). M, meniscus; AC, articular cartilage; B, bone; mm, micromass.
Techniques Used: Positive Control, Staining, Negative Control
Figure Legend Snippet: IHC of COL X shows the presence of weak signal in week 3 and week 4 micromass cartilage (a), whereas apoptosis assays show no apoptosis in micromass cartilage (b). Mouse decalcified P1 limb showed hypertrophic chondrocytes zone (arrows) as positive control for COL X (a). Mouse involuting MG (mammary gland) at postpartum day 4 showed apoptotic cells in mammary epithelium (arrow) that served as positive control, whereas no apoptotic cell was found at week 4 in micromass cartilage compared with positive control. mm, micromass.
Techniques Used: Positive Control
Figure Legend Snippet: IHC showed that micromass cartilage expressed aggrecan and COL VI at all the time points (weeks 1–4, a, b). Articular cartilage of rat knee expressed aggrecan and served as positive control (a). Human tracheal cartilage served as positive control for COL VI and showed that COL VI is pericellular in location in native cartilage (b). Higher magnification of week 4 bioengineered micromass cartilage also shows the pericellular localization of COL VI (arrowheads, b). Rat knee (a) and micromass cartilage (b) were used as negative control with rabbit IgG polyclonal and without primary antibody. M, meniscus; AC, articular cartilage; B, bone; mm, micromass.
Techniques Used: Positive Control, Negative Control